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lenticrispr v2 shrna constructs  (Addgene inc)


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    Structured Review

    Addgene inc lenticrispr v2 shrna constructs
    Lenticrispr V2 Shrna Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lenticrispr+v2+shrna+constructs/lentiCRISPR+v2+(Plasmid+%2352961)/pmc12467950-39-17-25
    Average 96 stars, based on 6137 article reviews
    lenticrispr v2 shrna constructs - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    shRNA:

    Article Title: Inhibiting UNC13B Suppresses Cell Proliferation by Upregulating the Apoptotic Pathway in Multiple Myeloma
    Article Snippet: To investigate the functional role of UNC13B, two short hairpin RNA (shRNA) sequences targeting UNC13B (GenBank accession no.: NM_020313.2 ) were designed and cloned into the lentiviral vector lentiCRISPR v2 (Addgene plasmid #52961). .. The shRNA target sequences were as follows: shUNC13B-1: 5′-GACCTACAAATGGGAGCAAAT-3′ shUNC13B-2: 5′-CGAGTCCTATGAGTTGCAGAT-3′ Lentiviral particles were generated by co-transfecting lentiCRISPR v2-shRNA constructs with the packaging plasmid psPAX2 (Addgene #12260) and the envelope plasmid pMD2.G (Addgene #12259) into human embryonic kidney 293T (HEK293T) cells using Lipofectamine 3000 (Thermo Fisher Scientific, Shanghai, China). .. Viral supernatants were collected at 48 and 72 h post-transfection, filtered (0.45 μm filter, Millipore, Shanghai, China; Cat#SLHV033RS), and used to transduce ARD multiple myeloma cells.

    Generated:

    Article Title: Inhibiting UNC13B Suppresses Cell Proliferation by Upregulating the Apoptotic Pathway in Multiple Myeloma
    Article Snippet: To investigate the functional role of UNC13B, two short hairpin RNA (shRNA) sequences targeting UNC13B (GenBank accession no.: NM_020313.2 ) were designed and cloned into the lentiviral vector lentiCRISPR v2 (Addgene plasmid #52961). .. The shRNA target sequences were as follows: shUNC13B-1: 5′-GACCTACAAATGGGAGCAAAT-3′ shUNC13B-2: 5′-CGAGTCCTATGAGTTGCAGAT-3′ Lentiviral particles were generated by co-transfecting lentiCRISPR v2-shRNA constructs with the packaging plasmid psPAX2 (Addgene #12260) and the envelope plasmid pMD2.G (Addgene #12259) into human embryonic kidney 293T (HEK293T) cells using Lipofectamine 3000 (Thermo Fisher Scientific, Shanghai, China). .. Viral supernatants were collected at 48 and 72 h post-transfection, filtered (0.45 μm filter, Millipore, Shanghai, China; Cat#SLHV033RS), and used to transduce ARD multiple myeloma cells.

    Construct:

    Article Title: Inhibiting UNC13B Suppresses Cell Proliferation by Upregulating the Apoptotic Pathway in Multiple Myeloma
    Article Snippet: To investigate the functional role of UNC13B, two short hairpin RNA (shRNA) sequences targeting UNC13B (GenBank accession no.: NM_020313.2 ) were designed and cloned into the lentiviral vector lentiCRISPR v2 (Addgene plasmid #52961). .. The shRNA target sequences were as follows: shUNC13B-1: 5′-GACCTACAAATGGGAGCAAAT-3′ shUNC13B-2: 5′-CGAGTCCTATGAGTTGCAGAT-3′ Lentiviral particles were generated by co-transfecting lentiCRISPR v2-shRNA constructs with the packaging plasmid psPAX2 (Addgene #12260) and the envelope plasmid pMD2.G (Addgene #12259) into human embryonic kidney 293T (HEK293T) cells using Lipofectamine 3000 (Thermo Fisher Scientific, Shanghai, China). .. Viral supernatants were collected at 48 and 72 h post-transfection, filtered (0.45 μm filter, Millipore, Shanghai, China; Cat#SLHV033RS), and used to transduce ARD multiple myeloma cells.

    Plasmid Preparation:

    Article Title: Inhibiting UNC13B Suppresses Cell Proliferation by Upregulating the Apoptotic Pathway in Multiple Myeloma
    Article Snippet: To investigate the functional role of UNC13B, two short hairpin RNA (shRNA) sequences targeting UNC13B (GenBank accession no.: NM_020313.2 ) were designed and cloned into the lentiviral vector lentiCRISPR v2 (Addgene plasmid #52961). .. The shRNA target sequences were as follows: shUNC13B-1: 5′-GACCTACAAATGGGAGCAAAT-3′ shUNC13B-2: 5′-CGAGTCCTATGAGTTGCAGAT-3′ Lentiviral particles were generated by co-transfecting lentiCRISPR v2-shRNA constructs with the packaging plasmid psPAX2 (Addgene #12260) and the envelope plasmid pMD2.G (Addgene #12259) into human embryonic kidney 293T (HEK293T) cells using Lipofectamine 3000 (Thermo Fisher Scientific, Shanghai, China). .. Viral supernatants were collected at 48 and 72 h post-transfection, filtered (0.45 μm filter, Millipore, Shanghai, China; Cat#SLHV033RS), and used to transduce ARD multiple myeloma cells.



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    96
    Addgene inc lenticrispr v2 shrna constructs
    Lenticrispr V2 Shrna Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lenticrispr+v2+shrna+constructs/lentiCRISPR+v2+(Plasmid+%2352961)/pmc12467950-39-17-25
    Average 96 stars, based on 1 article reviews
    lenticrispr v2 shrna constructs - by Bioz Stars, 2026-09
    96/100 stars
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